What are the uses of thin layer chromatography?
TLC is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance.
What is TLC used for in real life?
TLC can be used in forensic studies where body fluids, such as urine and blood can be tested for the presence of drugs. Acidic and neutral drugs can be identified by using octadecyl silica in the stationary phase, while plain silica and octadecyl silica can be used to identify basic drugs.
What is the example of thin layer chromatography?
Thin-layer chromatography is performed on a sheet of an inert substrate such as glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.
How is thin layer chromatography used in medicine analysis?
To analyze a substance, the plant material is extracted in solvent. A spot of this extract is placed near the bottom edge of the thin layer plate. Once the spot dries, the entire plate is placed into a solvent tank, with only the bottom of the thin layer plate submerged in the solvent.