THE EFFECT OF CRUDE
OF ALOE BARBADENSIS ON SOME HEMOSTATIC PARAMETERS OF FED ON THERMOXIDIZED PALM OIL
DIETS
CHAPTER ONE
INTRODUCTION
AND LITERATURE REVIEW
1.1 Introduction
1.2 Aims and objectives of the study
1.3 Justification of study
1.4 Scope of study
CHAPTER TWO
2.0 Literature
review
2.1 Photochemistry
of aloe Vera
2.2.1
General uses of aloe Vera
2.2.2 Theraperetic (medicinal) uses of aloe Vera
2.2.3 Anti-inflammatory
effects of aloe Vera
2.2.4 Laxative
effects of aloe Vera
2.2.5
Anti-cancer
properties of aloe Vera
2.2.6
Gastroprotective properties of aloe Vera
2.2.7 Anti
viral effects of aloe Vera
2.2.8 Wound healing properties of aloe Vera
2.2.9 Aloe
Vera gels effects on the immune system
2.2.10 Effects of aloe Vera on burns
2.2.11 Hypoglycemic
effects of aloe Vera
2.2.12 Aloe Vera in veterinary medicine
2.
3 Other
effects of aloe Vera
2.
3.1 Mechanism of action of aloe Vera
2.3.2
Mechanism of anti
inflammatory action of aloe Vera.
2.3.3 Mechanism
of laxative/ cathartic action of aloe Vera.
2.3.4 Mechanism
of wound healing action of aloe Vera
2..3.5 Side effects, contrain dication and toxicity
of aloe Vera.
2.4
Homeostasis
2.
4.1 Steps
of mechanism
2.4.2 Blood clotting factors
2.4.3 Sequence
of clotting mechanism
2.4.4 Bleeding time
2.4.5 Clotting time
2.4.6 Prothrombin time
2.4.7 Homeostasis disorders /
treatment
2.5 The
oil palm tree
2.5.1 Thermoxidized palm oil
2.5.2
Effects of thermoxidized palm oil on health
CHAPTER THREE
3.0 Materials and methods
3.1 Materials
3.1.1 Experimental
animals
3.1.2 Experimental
gel
3.1.3 Thermoxidized palm oil
3.2 Methods
3.2.1 Experimental
procedure
3.2.2 Preparation
of Experimental animal for the determination of homeostatic parameters
3.2.3 Determination of bleeding time by Duke’s
Method
3.3.3 Determination
of Clotting time
3.3.4 Determine
of Prothrombin time
3.3.5 Determine
of Platelet count
3.4 Precautions
3.5 Statistical Analysis
CHAPTER
FOUR
4.0 Result
4.1
Comparison of mean food intake in control, thermoxidized palm oil (T.P.O) and
T.P.O + aloe vera fed groups
4.2
Comparison of mean water intake in control, thermoxidized palm oil (T.P.O) and
T.P.O + aloe vera fed groups.
4.3 Comparison
of mean body weights of control, thermoxidized palm oil (T. P. O) and T.P.O
aloe vera fed groups.
4.4
Comparison of bleeding time in control, thermoxidized palm oil (T.P.O)
and T.P.O + aloe vera extract fed groups.
4.5 Comparison of clotting time in control, thermoxidized
palm oil (T.P.O) and T.P.O + aloe vera extract fed groups.
4.6 Comparison
of prothrombin time in control, thermoxidized palm oil (T.P.O) and T.P.O + aloe
vera extract fed groups.
4.7 Comparison
of platelet count in control, thermoxidized palm oil (T.P.O) and T.P.O + aloe
vera extract fed groups.
CHAPTER
FIVE
4.0 Discussion
and conclusion
4.1 Discussion
4.2 Conclusion
CHAPTER ONE
INTRODUCTION AND LITERATURE REVIEW
1.0
Introduction
Aloe barbadensis or aloe Vera is a
succulent plant from the family “Liliaceae”, it originated in the
African content. The genus has many common names and is often referred to as
aloe vera, lily of the desert, burn plant, the plant of immortality, first aim
plant, wand of heaven and medicinal plant. The name is derived from the Arabic
word “Alloeh” meaning “shining bitter substance”. The Genus
contains at least 324 species of herbs, shrubs and ;s (cross white and cross
white, 1984). Aloe vera is a perennial with 15-30 fleshy leave up to 0.5m long
and 8-Pcrn across the base. Saw like teeth mark the margins- of leaves
(Grindlay and Reynolds, 1986). Aloe vera plants withstand high temperatures
and long periods drought, due to their ability to
store water in their succulent leaves. However, freezing temperatures can
damage or kill the plant. Medically and non- medically, aloe vera has been used
for several thousands of years in different cultures from ancient Egypt to
Greece, Rome to China, India and Africa (crosswhite and crosswhite, 1984;
Grindlay and Reynolds, 1986).
In the first century, C.E, the Greek physician, Dioscorides used
aloe Vera for mouth infections, sores, wounds and as purgatives. Egyptians,
Assyrians and Mediterranean peoples used the latex primarily and the gel as a
purgative. The plant was used by the Arabs, Spaniards, ancient Greeks and
persians and is still in use by hunters in Africa to reduce perspiration and
body scent.
In 500 B.C, Egyptians recorded the use of
aloe vera in treating burns, parasites and infections. The plant was called.
the plant of immortality” by the Egyptians because it can live and even
bloom without soil and was given as an offering at the funerals of pharaohs. It
was also used in the baths of the Egyptian queens Nefertiti and Cleopatra to
keep their skin soft and young (Pamplona Roger, 2001). Today, Egyptians still
hang an aloe vera plant over the door of a house to
provide a long
and fruitful life
for its occupants. In India,
the plant is
used as cathartic, anthelminthic, emmenagogue
and stomachic. Aloe
vera latex was used before 1930s in the united states as laxatives ton,
1961; crosswhite and crosswhite, 1984; Grindlay and Reynolds, 1986; Evens
1996).
1.2Aims and objective s of the study
The aim of this study is to ascertain the effect of crude of aloe
barbadensis (aloe vera ) on some hemostatic parameters of fed on thermoxidized
palm oil diets. The objective is to ascertain if
aloe vera has any
effect on hemostatic derangements that may result from
thermoxidized palm oil diet.
1.3 Justification of
study
It has been known that aloe vera has anti-inflammatory,
laxalive,
anti-hypertensive, anticancer, hypoglycaemic fects etc but not much work has been done on its effects
on Hemostasis, especially in rats placed on a diet
mixedwith thermoxidized palm oil. This research work is
therefore aimed at elucidating its effect on Hemostatic parameters ofrats fed on thermoxidized
palm oil diets.
1.4 SCOPE
OF THE STUDY
The scope of the study involves measuring bleeding time, clotting time,
prothrombin time and platelet count in 5 albino wistar rats fed with pellet
mixed with thermoxidised palm oil and also 5 albino wistar rat fed with the
same mixed pellet and in addition 0.1ml/100g body weight of refined aloe vera gel orally administered
for four weeks (28 days)and comparing the results with control group (5 albino
wistar rats) fed only on normal pellet for same period.